Establishment of Cedrus atlantica Manetti karyotype
General Information:
Master’s |
Level |
Establishment of Cedrus atlantica Manetti karyotype |
Title |
Cell and molecular biology |
Specialty |
Cover Page:
Outline:
Introduction
Part I: Literature review
I. Cytogenetic
I.1. Preview
I.2.Plant cytogenetic interest
I.3. Chromosomes
I.3.1. Chromosomes compositions
I.3.2. Chromosomes identification criteria
I.3.3. Chromosomes types
I.3.3.1. Metacentric
I.3.3.2. Sub-metacentric
I.3.3.3. Accrocentri
I.3.3.3.Telocentri c
I.3.4. Chromosomes marking techniques
I.5. Techniques for determining variability (polymorphisme) in plants species
I.5.1. Morphological variability
I.5.2. Biochimical variability
I.5.3. Molecular variability
I.5.3.1. Genetic variability of molecular markers
I.5.3.2. Genetic variability phenotypic traits
I.5.4. Chromosomal variability
II. Atlas cedar
I.1. Preview on Cedrus genus
I.2. Cedrus atlantica Manett
I.2.1. Systematic
I.2.2. Botanical characters
I.2.2.1.Architecturalunit
I.2.2.2.Shape
I.2.2.3.Twigs
I.2.2.4. Crust
I.2.2.5.Needles (leaves)
I.2.2.6. Flowers
I.2.2.7.Cones (fruit)
I.2.2.8. Seeds
I.2.2.9.Roots
I.2.3. Distribution area of Cedrus atlantica
I.2.3.1. Natural area
I.2.3.2. Introduction area
I.2.4. Cedrus atlantica interest
I.2.4.1. Ecological interest
I.2.4.2. Medicinal interest
I.2.4.3. Industrial interest
Part II: Methodology
I. Material and methods
I. Objective
II. Material and methods
II.1. Plant material used
II.2. Samples sites
II.3. The used seeds
III. Method
III.1. Karyotype establishment
III.1.1.Disinfection and put in germination of seeds
III.1.2. Pre-treatment
III.1.3. Fixation
III.1.4. Hydrolysis
III.1.5. Staining
III.1.6.Montage
III.1.7. Observation
II. Results and discussion
I.1. Results and discussion
III. Conclusion and perspectives
I.1. Conclusion
I.2. Perspectives
